Microfluidic Lab-on-a-chip System with Integrated Sample Preparation for Processing Immunoassays

We present an advanced, injection molded microfluidic lab-on-a-chip cartridge to process immunoassays with unit operations for sample preparation, metering, mixing, incubation, washing, chemiluminescence detection and waste handling. A newly developed readout device is capable of recording data points under rotation. Latest assay results feature a lower average standard deviation (7 % of maximum signal) in contrast to previously achieved 27 % [1]. A competitive chemiluminescent Estradiol immunoassay is demonstrated on-chip with a series of different Estradiol concentrations. A lower limit of detection of 60 pg/mL is established with a time-to-result of 45 min compared to 60 min in a microtiterplate.